Biosynthesis of Zn-nano complex using olive seeds for antimicrobial regulation of nanostructured materials

Dandruff affects a large number of people throughout the world. Microsporum spp. and Nannizzia spp. are fungal species connected with dandruff because they increase keratinocyte cytokine production (epidermal cells synthesizing keratin). Because dandruff is such a bothersome problem for individuals, many efforts are being made to resolve this issue. This study focused on the synthesis of the free ligand (E)-N-(4,6-dimethy1pyrimidin-2-yl)-4-(((2-hydroxynaphtha1en-1-yl) methylene) amino) benzenesulfonamide by reacting 1‑hydroxy-2-naphthaldehyde and amino-N-(4,6-dimethy1pyrimidin-2-yl) 4benzenesulfonamide. Its complex with Zn (II) was also biosynthesized as a nano complex in the presence of olive seed extracts. The nano complex was characterized by different methods such as elemental analyses, FT-IR, and SEM. The olive seeds were collected, shade-dried, powdered, and aquas extracted. The results observed that the same MIC value of 12.5 mg/ml was recorded for Microsporum canis and Microsporum gypseum. However, a lower MIC of 6.25 mg/ml was recorded for Nannizzia fulva. On the other hand, the MIC values recorded for Zn-nano complexes were lower than those recorded for the olive seed extract. These values were recorded as 6.25 mg/ml for Microsporum canis compared with 3.12 mg/ml for both Microsporum gypseum and Nannizzia fulva. Well, a diffusion experiment was used to test the antifungal effects of biosynthesized nano complex. It was found that combining these nano complexes with shampoo boosts the anti-fungal impact against dandruff-causing fungi (Microsporum spp. and Nannizzia spp.). This study demonstrates a simple, quick, and cost-effective method to bio-synthesis an effective nano-based complex with enhanced anti-fungal capabilities against dandruff-causing fungi.