Diagnosis of Lymphatic Filariasis Using Nano-Chip CA-RD Assay Compared To Nano-Based Enzyme Linked Immunosorbent Assay

Filariasis is a parasitic infection caused by small roundworms that live in humans' blood and tissues. This
study developed a modified nano enzyme-linked immunosorbent test (ELISA) for detection of filarial
antigen in human sera. The assay's limit of detection (LOD) and cost were dramatically decreased as a
result of this improvement. Serum samples from affected persons were gathered across Egypt's
governorates. Membrane filtration was used to determine the individuals' microfilarial status, which was
then examined under a light microscope. Sera were collected from 27 microfilariae patients (MF), 21 with
chronic lymphatic illness (CL), and 20 microfilariae negative healthy people living in non-endemic areas, as
well as sera from 20 other parasites affected patients, after informed consent was obtained. Following
their consent, all of these individuals underwent the following procedures: history taking, clinical
examination, and laboratory investigations, which included examination of blood samples for microfilaria
using thick blood film and serological tests for detection of the circulating filarial antigen (CFA) using
sandwich ELISA, Home-made rapid card test, and nano-based ELISA. All non-endemic normal sera were
negative for filarial antigen using different applied techniques. On the other hand, 23/27 microfilaraemia
sera and 7/21 chronic lymphatic patients' sera were found using Home-made rapid card test. Filarial
antigen was found in the serum of 18/27 microfilaraemia patients and 9/21 chronic lymphatic patients
using sandwich ELISA. Filarial antigen was found in 25/27 microfilaraemia sera and 6/21 chronic lymphatic
patients' sera using nano-based ELISA. Finally, utilizing a modified enzyme-linked immunosorbent assay
(ELISA) approach, compinednanoparticle are effective nano-analytical tools for detecting human
filariasis.In addition home-made rapid card test was effective diagnostic method for filariasis.