An approach of fungal aerospora in students class-rooms: Identification, biocontrol and its pathogenicity

According to reports, one of the key elements influencing how people's health is affected in classrooms is the air quality.
In the current investigation, pathogenicity of the dominant fungus was evaluated, and the goal was to isolate and identify
indoor air fungi dispersed in classrooms.In pure cultures, 11 from 7 genera were isolated and identified. The most
isolated fungus, Trichoderma longibrachiatum (T. longibrachiatum), was eradicated using essential oils (camphor, mint,
basil, lavender, rose, and lemon). To determine the pathogenicity of T. longibrachiatum on rabbit hematological,
biochemical, and histopathological features, oral administration of 50 mg/kg of the organism for two weeks was
conducted.Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Cladosporium cladosporioides, Penicillium
frequentus, Penicillium purporogenum, Paecilomyces lilacinus, Paecilomyces variotii,
Rhizopus stolonifer, T.
longibrachiatum and Ulocladium sp. were isolated from twelve class rooms (CMs). Air drenched with each of camphor
and mint oil suppressed T. longibrachiatum growth by 100% as compared to the control. While, basil, lavender, rose and
lemon oil did not show any antifungal activity. T. longibrachiatum oral administration induced a significant decrease in
RBCs count, Hb concentration, MCV and MCH values. A remarkable reduction of WBCs count and Neutrophil were
observed. Moreover, T. longibrachiatum induced a significant elevation of hepatic biomarkers (ALT and AST), No
variations were noticed in the values of albumin, LDH activity and Creatinine. Histopathological assay revealed a hepatic
and pulmonary deterioration subsequently to T. longibrachiatum administration. Taken together, our results showed that
T. longibrachiatum was the most prevalence isolated fungi from the classrooms indoor air and exerting various biological
hazards which requires purifying classrooms indoor air using the natural essential oils.