Skip to main content

Anticancer effect of Citrullus colocynthis and Capparis spinosa against human Cervix and Hepatocellular cancer cell lines

Author name : HAIFA ABDULAZIZ SAKET ALHAITHLOUL
Publication Date : 2022-01-28
Journal Name : Ecology, Environment and Conservation, ISSN# 0971-765X-quarterly.

Abstract

The current work aims to assess the anticancer effect of Citrullus colocynthis, Capparis spinose against human Cervix (Hela) and hepatocellular (Huh7) cancer cell lines and understand the mode of action at molecular level via gene expression and cell cycle analysis. The Methanolic extracts were prepared. Antioxidant activities and Phytochemical screening of every extract were estimated. To measure cytotoxicity, MTT test was employed, and Annexin/propidium iodide (PI) staining was used to measure apoptosis induction and change in gene expression level was detected by Real Time PCR (qPCR). Obtained findings explained anticancer potential of both Citrullus colocynthis and Capparis spinosa methanolic extracts against cervical and hepatocellular carcinoma. The anticancer effect of Citrullus colocynthis was higher than Capparis spinosa extract. This effect might be due to the role TPC and TFC through induction of apoptotic pathways influenced by upregulation of p3 and p21 genes. These results may help expand the importance of the Citrullus colocynthis and Capparis spinosa; and may be regarded in the detection of new anticancer compounds.

Keywords

Anticancer, Citrullus colocynthis, Capparis spinose, Human Cervix, Hepatocellular cancer

Publication Link

https://doi.org/10.53550/EEC.2022.v28i01s.081

Block_researches_list_suggestions

Suggestions to read

HIDS-IoMT: A Deep Learning-Based Intelligent Intrusion Detection System for the Internet of Medical Things
Ahlem . Harchy Ep Berguiga
Generalized first approximation Matsumoto metric
AMR SOLIMAN MAHMOUD HASSAN
Structure–Performance Relationship of Novel Azo-Salicylaldehyde Disperse Dyes: Dyeing Optimization and Theoretical Insights
EBTSAM KHALEFAH H ALENEZY
“Synthesis and Characterization of SnO₂/α-Fe₂O₃, In₂O₃/α-Fe₂O₃, and ZnO/α-Fe₂O₃ Thin Films: Photocatalytic and Antibacterial Applications”
Asma Arfaoui
Contact