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Quantitative assessment of phosphate food additive in frozen and chilled chicken using spectrophotometric approach combined with graphitic digestion

Author name : Ibrahim hotan zaidi alsohaimi
Publication Date : 2022-04-25
Journal Name : Food Chemistry

Abstract

A new method based on spectrophotometry combined with graphitic digestion was developed for quantitative assessment of phosphate in frozen and chilled chicken meat. Digestion reagents comprising HNO3 (conc.) & H2SO4 (conc.) and HNO3 (5 M) & H2SO4 (conc.) were found to have optimal composition, affording similar recovery values of 100% and 99%, respectively, with excellent linearity (R2 > 0.999) and good limit of detection (LOD = 0.032 mg/L) and limit of quantification (LOQ = 0.10 mg/L), whereas other reagents offered lower re- coveries (0–1.73%). Phosphate was found in concentrations of 3.38–5.90 g/kg and 3.96–26.94 g/kg in frozen and chilled chicken, respectively. Chilled chicken contained higher amounts of phosphate (>20 g/kg) than recommended by the European Commission (EC, 5 g/kg), either alone or in a mixture of processed meat products. This method is simple, cost-effective, and can be used as an alternative for analyzing phosphate in various samples comprising a similar matrix.

Keywords

Chicken, Phosphate, Nitric acid, Sulfuric acid, Graphitic digestion, Colorimetric, Spectrophotometer

Publication Link

https://doi.org/10.1016/j.foodchem.2022.133050

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