LC/MS Profiling and Gold Nanoparticle Formulation of Major Metabolites from Origanum majorana as Antibacterial and Antioxidant Potentialities

Origanum majoranum L. is a Lamiaceae medicinal plant with culinary and ethnomedical applications. Its biological and phytochemical profiles have been extensively researched. Accord‐ ingly, this study aimed to investigate the chemical composition and the antibacterial and antioxi‐ dant properties of O. majoranum high features, as well as to search for techniques for activity opti‐ mization. A metabolomics study of the crude extract of O. majoranum using liquid chromatography‐ high‐resolution electrospray ionization mass spectrometry (LC ± HR ± ESI ± MS) was conducted. Five fractions (petroleum ether, dichloromethane, ethyl acetate, n‐butanol, and aqueous) were de‐ rived from the total extract of the aerial parts. Different chromatographic methods and NMR anal‐ ysis were utilized to purify and identify the isolated phenolics (high features). Moreover, the anti‐ microbial, antibiofilm, and antioxidant activity of phenolics were performed. Results showed that metabolomic profiling of the crude extract of O. majoranum aerial parts revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the isolation and identification of seven high‐feature compounds comprising two phenolic acids, rosmarinic and caffeic acids, one phenolic diterpene, 7‐methoxyepirosmanol, in addition to four flavonoids, quercetin, hesperitin, hesperidin, and luteolin. On the other hand, 7‐methoxyepirosmanol (OM1) displayed the most an‐ timicrobial and antioxidant potential. Such a phenolic principal activity improvement seems to be established after loading on gold nanoparticles.