Designing a highly immunogenic multi epitope based subunit vaccine against Bacillus cereus

Objective: Serious non-gastrointestinal-tract infections and food poisoning are caused by Bacillus cereus.
Vaccination against B. cereus is very important. The aim of this study was to identify and analyze B and T
cell epitopes for chromate transporter protein of the bacteria.
Methods: Multiple sequence alignment with the Clustal Omega method was used to identify conserved
regions and Geneious Prime was used to produce a consensus sequence. T and B cell epitopes were pre
dicted by various computational tools from the NetCTL and Immune Epitope Database (IEDB), respec
tively.
Results: Altogether, 6 HTL cells and 11 CTL epitopes were predicted. This vaccine’s molecular docking is
done with Patch Dock and LigPlot to verify interactions. The immune server (C-IMMSIM) was used to
develop In silico immune response in order to assess the multi-epitope vaccine’s immunogenic profile.
Conclusion: We designed universal vaccine against B. cereus responsible for food poisoning. The disease
may be avoided with the aid of the proposed epitope-based vaccine.