Therapeutic Effect of Sepia pharaonis Ink Extract and Ellagic Acid on the Toxicity Induced by Cyclophosphamide in Male Albino Rats

The combination between anticancer drugs and natural products has great attention aiming at exploiting their efficiency and reducing systemic toxicity. Subsequently, the current study was constructed to evaluate the potential curative role of crude Sepia ink extract (SIE) and Ellagic acid (EA) against cyclophosphamide (CP) toxicity. Male albino rats were divided into five groups (n = 10). All groups except group I received CP. Group I served as a control group, group II received a single dose of CP (200 mg/kg, i.p.) and served as CP group, rats of group III received CP + SIE (200 mg/kg body weight), group IV received CP+ ‏ EA (60 mg/kg) and group V received CP + SIE (200 mg/kg body weight) + EA (60 mg/kg). Data showed that CP administration significantly decreased RBCs, WBCs, PLTs count, PCV%, and Hb content. Post administration of SIE and EA caused a significant elevation in the hematological values. Also, SIE and EA treated groups reduced the hepatic and renal toxicity induced by CP treatment, where liver and kidney functions (AST, ALT, ALP, creatinine, urea, and uric acid) significantly decrease. On the other hand, serum albumin and T. protein levels increased compared to CP-treated groups. The current results revealed that CP induced hematological alterations and various tissues injury. Natural products as SIE and EA eliminated the CP toxicity.