Vitamin D Receptor Polymorphisms among Sudanese Females with Breast Cancer attending Radio and Isotope Center in Khartoum, Sudan

ackground: Vitamin D Receptor (VDR) gene polymorphisms has been reported to
be associated with different cancers, including breast cancer. geographical and
environmental factors have an impact on vitamin D (VD) metabolism. This study
aims to evaluate VDR-gene polymorphisms (Fok1, Bsm1, Taq1, Apa1) and its
association with breast cancer risk among Sudanese females.
Materials and Methods: this is a case control study done in the Radio and Isotope
Center in Khartoum, Sudan. Hundred thirty seven patients and 69 healthy controls
were enrolled after informed consent. Blood genomic DNA was extracted using
quinidine chloroform method, and single nucleotide polymorphisms (SNPs) Fok1
(T/C) [rs2228570], Bsm1 (G/A) [rs1544410], Taq1 (C/T) [rs731236] and Apa1 (C/A)
[(rs7975232] in VDR-gene were detected by PCR and restriction fragment length
polymorphism (PCR-RFLP) analysis.