Stabilization of glutathione redox dynamics and CYP2E1 by green synthesized Moringa oleifera-mediated zinc oxide nanoparticles against acrylamide induced hepatotoxicity in rat model: Morphometric and molecular perspectives

The terrible reality is that acrylamide (AA) is a common food contaminant found in a wide variety of commonly
consumed foods. This research involves the advancement of a more dependable technique for the bio-fabrication
of zinc oxide nanoparticles (ZNPs) through the green method using Moringa Oleifera extract (MO-ZNPs) as an
efficient chelating agent for acrylamide (AA). The effects of AA on glutathione redox dynamics, liver function,
lipid profile, and zinc residues in Sprague Dawley rats are investigated. Finally, the microarchitecture and
immunohistochemical staining of Caspase-3 and CYP2E1 were determined in the liver tissue of rats. Four
separate groups, including control, MO-ZNPs (10 mg/kg b. wt), AA (20 mg/kg b. wt), and AA + MO-ZNPs for 60
days. The results revealed a suppressed activity of glutathione redox enzymes (GSH, GPX,and GSR) on both
molecular and biochemical levels. Also, AA caused elevated liver enzymes, hepatosomatic index, and immunohistochemical staining of caspase-3 and CYP2E1 expression. MO-ZNPs co-treatment, on the other hand, stabilized glutathione-related enzyme gene expression, normalized hepatocellular enzyme levels, and restored
hepatic tissue microarchitectures. It could be assumed that MO-ZNPs is a promising hepatoprotective molecule
for alleviating AA-induced hepatotoxicity. We witnessed changes in glutathione redox dynamics to be restorative. Glutathione and cytochrome P450 2E1 play crucial roles in AA detoxification, so maintaining a healthy
glutathione redox cycle is necessary for disposing of AA toxicity.