Assessment of the Effectiveness of Toxoplasma Surface Antigen Grade I for Diagnosis of Human Toxoplasma gondii

Background: Toxoplasmosis represents a neglected equatorial
poverty disorder triggered by an intracellular mandatory protozoan
parasite, known as Toxoplasma gondii.
Aim of the work: The purpose of this study was to assess the
Effectiveness of Toxoplasma Surface Antigen Grade I for diagnosis
of human Toxoplasma gondii in Egypt by Sandwich ELISA
Technique.
Subjects and Methods: This study was conducted on 94
individuals, divided into 3 categories, category I: Toxoplasma
gondii, category II: Other parasites, it encompassed 10 infected
patients that have E. histolytica and 14 infected with G. lamblia, and
category III: Healthy control group.
Results: The cutoff value was 0.233 when detecting Toxoplasma
(SAG1).The serum findings appear positive in 43 cases (86 percent)
of category I, whilst 7 cases (14 percent) appear negative. In
category II (patient with different parasites): 10 positive cases of
Entamoeba histolytica have been confirmed, while the other 14
cases were positive with G. lamblia. All healthy control cases
(category III) were negative. The sensitivity was 86% However; the
specificity was observed at 81.81%.
Conclusion: From the obtained results, we can conclude that: to
evaluate the different immunodiagnostic antigens detection assays,
choice, and purification of the suitable antigen, accompanied by the
manufacture and purification of its particular antibodies, are
mandatory. The employment of rabbit anti-Toxoplasma gondii IgG
polyclonal antibodies in sandwich ELISA techniques for the
identification of SAG1 in human serum provides a sensitive and
specific tool for immunodiagnosis in human toxoplasmosis.