Enhancement of Annexin V in response to combination of epigallocatechin gallate and quercetin as a potent arrest the cell cycle of colorectal cancer

Colorectal cancer (CRC) is one of the most common cancers leading to comorbidities and mortalities globally.
The rational of current study was to evaluate the combined epigallocatechin gallate and quercetin as a potent
antitumor agent as commentary agent for therapeutic protocol. The present study investigated the effect of
epigallocatechin Gallate (EGCG) (150mg) and quercetin (200mg) at different proportions on proliferation and
induction of apoptosis in human colon cancer cells (HCT-116). Cell growth, colonogenic, Annexin V in addition
cell cycle were detected in response to phytomolecules. Data obtained showed that, the colony formation was
inhibited significantly in CRC starting from the lowest concentration tested of 10 μg/mL resulting in no colonies
as visualized by a phase-contrast microscope. Data showed a significant elevation in the annexin V at 100 μg/mL
EGCG(25.85%) and 150 μg/mL quercetin (48.35%). Moreover, cell cycle analysis showed that this combination caused
cell cycle arrest at the G1 phase at concentration of 100 μg/mL (72.7%) and 150 μg/mL (75.25%). The combined effect
of epigallocatechin Gallate and quercetin exert antiproliferative activity against CRC, it is promising in alternative
conventional chemotherapeutic agent