Detection of Helicobacter Pylori from Stomach Biopsies: A Rapid Culture Method

Objective: To identify a rapid culture method capable of isolating and detecting H. pylori from stomach biopsies. Study Design: Descriptive / cross-sectional study Place and Duration of Study: This study was conducted at the Omdurman Islamic University, Sudan from 1 st November 2021 to 30 th August 2022. Materials and Methods: The stomach biopsy specimens from 40 patients were collected and centrifugation was done in phosphate buffer saline at 3000 rpm for two minutes. The culture was performed on Modified Brain Heart Urea Agar (MBHUA) media by placing two to three drops of sediment. The cultures were incubated at 37°C in microaerophilic conditions using a candle jar for up to 5 days and observed for growth and change in the color of the medium daily. Results: Modified Brain Heart Urea Agar media allowed for rapid detection of urease activity in H. pylori within 24 hours after its addition. The bacterial growth was observed three days following the incubation of cultured plates under microaerophilic conditions provided by a candle jar. These results develop rapid detection of H. pylori with clear growth. Conclusion: H. pylori can be detected more rapidly in modified brain Heart urea agar than the other H. pylori growth media.