Effect of Laser Light on Growth, Physiology, Accumulation of Phytochemicals, and Biological Activities of Sprouts of Three Brassica Cultivars

Brassica sprouts are known as a good source of antimicrobial bioactive compounds such as phenolics and
glucosinolates (GLs). We aim at understanding how He−Ne laser light treatment (632 nm, 5 mW) improves sprout growth and
physiology and stimulates the accumulation of bioactive metabolites in three Brassica spp., i.e., mustard, cauliflower, and turnip.
Moreover, how these changes consequently promote their biological activities. Laser light improved growth, photosynthesis, and
respiration, which induced the accumulation of primary and secondary metabolites. Laser light boosted the levels of pigments,
phenolics, and indole and aromatic precursors of GLs, which resulted in increased total GLs and glucoraphanin contents. Moreover,
laser light induced the myrosinase activity to provoke GLs hydrolysis to bioactive sulforaphane. Interestingly, laser light also reduced
the anti-nutrient content and enhanced the overall biological activities of treated sprouts including antioxidant, antibacterial, antiinflammatory, and anticancer activities. Accordingly, laser light is a promising approach for boosting the accumulation of beneficial
metabolites in Brassica sprouts and, subsequently, their biological activities.