تجاوز إلى المحتوى الرئيسي

Antibacterial and cytotoxic potency of thermophilic Streptomyces werraensis MI?S.24?3 isolated from an Egyptian extreme environment

Author name : MOHAMMED RAWWAY KHALIL KHLAF
Publication Date : 2021-07-14
Journal Name : Archives of Microbiology

Abstract

The need for novel and active antibiotics specially from actinomycetes is essential due to new and drug-resistant pathogens.
In this study, 87 actinomycetes were isolated, and 18 strains among them characterized as thermophilic actinomycetes.
Further fractionation and preliminary antibacterial activities indicated that one strain, coded as MI-S.24-3, showed good
antibacterial activity. Based on the phenotypic, genomic, phylogenetic, and biochemical analyses, MI-S.24-3 was identified as
Streptomyces werraensis. Results demonstrated that the ethyl acetate active fraction showed maximum antibacterial activity
against Staphylococcus aureus and Escherichia coli with MIC (12.7 ± 0.1 and 18.3 ± 0.2 mg/mL), and MBC (96.5 ± 1.4 and
91.5 ± 0.7 mg/mL), respectively, with determination of time kill kinetics assay. The active fraction showed moderate-to-weak
cytotoxic effects against human lung carcinoma (A549 cells), breast cancer cell line (MCF-7), and human cervical carcinoma
(HELA cells) with a IC50
of (23.8 ± 1.2, 54 ± 1.8, 96.4 ± 3.2 μg/mL, respectively). Active components were characterised by
different chemically volatile, ester, and lactone compounds, determined by GC–MS coupled with daughter ions of (GC–MS/
MS). Notably, erucic acid and reynosin identified compounds are rare metabolites produced by Streptomyces werraensis.
Our findings demonstrated that the MI-S.24-3 strain could be a potential source for active compounds of biomedical and
pharmaceutical interest.

Keywords

Thermophilic · Streptomyces werraensis · Antibacterial · Cytotoxic activity · GC–MS

Publication Link

https://doi.org/10.1007/s00203-021-02487-0

Block_researches_list_suggestions

Suggestions to read

HIDS-IoMT: A Deep Learning-Based Intelligent Intrusion Detection System for the Internet of Medical Things
Ahlem . Harchy Ep Berguiga
Generalized first approximation Matsumoto metric
AMR SOLIMAN MAHMOUD HASSAN
Structure–Performance Relationship of Novel Azo-Salicylaldehyde Disperse Dyes: Dyeing Optimization and Theoretical Insights
EBTSAM KHALEFAH H ALENEZY
“Synthesis and Characterization of SnO₂/α-Fe₂O₃, In₂O₃/α-Fe₂O₃, and ZnO/α-Fe₂O₃ Thin Films: Photocatalytic and Antibacterial Applications”
Asma Arfaoui
تواصل معنا