New 4-imino-4H-Chromeno[2,3-d] Pyrimidin-3(5H)-Amine: Synthesis, Cytotoxic Effects on Tumoral Cell Lines and in Silico ADMET Properties

The synthesis of new 4-imino-4H-chromeno[2,3-d]pyrimidin-3(5H)-amine in four steps including one step under microwave dielectric heating is re- ported. The structural identity of the synthesized compounds was established according to their spectroscopic analysis, such as FT-IR, NMR and mass spec- troscopy. These new compounds were tested for their antiproliferative activi- ties on seven representative human tumoral cell lines (Huh7 D12, Caco2, MDA-MB231, MDA-MB468, HCT116, PC3 and MCF7) and also on fibro- blasts. Among them, only the compounds 6c showed micromolar cytotoxic activity on tumor cell lines (1.8 < IC50 < 6 μM) and no toxicity on fibroblasts (IC50 > 25 μM). Finally, in silico ADMET studies ware performed to investigate the possibility of using of the identified compound 6c as potential anti-tumor compound.