Therapeutic potential of stromal vascular fraction in early diabetic nephrotoxicity: a Histomorphometric and immunohistochemical analysis in type 2 diabetes rat model.

Background: Diabetic nephropathy (DN), a common complication of type 2 diabetes (T2D),
significantly contributes to end-stage kidney disease (ESKD). Despite conventional treatments
aimed at slowing disease progression, there is a pressing need for novel therapies. This study
evaluates the potential therapeutic impact of adipose tissue derived stromal vascular fraction
(SVF) on early diabetic nephrotoxicity in a rat model.
Materials and methods: Thirty-one male albino rats were divided into control and diabetic
groups, with the latter further split into untreated (T2Da) and SVF-treated (T2Db) subgroups.
Biochemical, histological, immunohistochemical, and morphometric analyses were conducted.
Results: We demonstrated that SVF treatment reduced oxidative stress, lowered serum
creatinine, and improved renal architecture by mitigating fibrosis and cellular infiltration,
suggesting enhanced tissue regeneration and reduced inflammation. SVF also facilitated cellular
repair, indicated by increased endothelial cell proliferation and reduced glomerular damage.
Conclusions: This study underscores SVF's potential as a promising regenerative approach for
managing early-stage DN, warranting further research to elucidate its mechanisms.